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en:construction:hotte_a_flux_laminaire [2013/04/03 22:19] ph [Overview of cuts] |
en:construction:hotte_a_flux_laminaire [2013/04/23 14:22] ph [Laminar flow hood] |
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- | <WRAP hide>====== Laminar flow hood</ | + | <wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish either at the time the lid is opened (during pouring or inoculation) or during incubation »</ |
- | <WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https:// | + | |
- | Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http:// | + | <tab> |
+ | ====== Laminar flow hood ====== | ||
+ | The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures. | ||
====== Prototype 1 & 2 ====== | ====== Prototype 1 & 2 ====== | ||
The first prototype met the expectations, | The first prototype met the expectations, | ||
- | < | + | < |
- | + | ||
- | <WRAP box 61% center> | + | |
- | <WRAP clear></ | + | |
+ | <WRAP centeralign> | ||
===== Improvements ===== | ===== Improvements ===== | ||
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| **1** |Stirrer | | **1** |Stirrer | ||
| **2** |Stirrer-motors | | **2** |Stirrer-motors | ||
- | | **4** |Stirrer-front stands | + | | **6** |Stirrer-front stands |
| **2** |Stirrer-rear stands | **SFc** | | **2** |Stirrer-rear stands | **SFc** | ||
</ | </ | ||
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|Fan (Fasco™ A1200 + bracket)| | |Fan (Fasco™ A1200 + bracket)| | ||
|HEPA Filter (18" | |HEPA Filter (18" | ||
- | |Pre-filters (9 ¾"x19 ¾" | + | |Pre-filters (9 ¾"x19 ¾" |
|3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(: | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(: | ||
|Power cord (115V, 15A)| 1 | 10 | **10** | |Power cord (115V, 15A)| 1 | 10 | **10** |