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Introduction

— Home
— Introduction

Agar culture

— Preparation
— Transplanting
— Culture of spores, storage

Indoor cultivation

Vegetative
— Substrate preparation
— Liquid culture, incubation

Generative
— Flowering, harvest
— Spores harvest

Outdoor cultivation

— Stumps and logs
— Establishment of a parcel
— Propagation
— Harvest

Construction

★ Laminar flow hood
— Flask for liquid culture
— Blooming box
— Bioreactor

Appendices

★ Recipes calculator
— Equipement and suppliers

About

★ Donate

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en:construction:hotte_a_flux_laminaire [2013/04/09 21:04]
ph 		en:construction:hotte_a_flux_laminaire [2013/04/27 11:47]
ph
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-<WRAP hide>====== Laminar flow hood</WRAP> +<wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish at the time the lid is opened; during pouring, inoculation or incubation »</wrap>[(:ref:stamets_ggm)]
-<WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=6HLVNSG9CPQYJ|financial contribution]], regardless of the amount, would be greatly appreciated.+
  
-Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http://creativecommons.org/licenses/by-nc-sa/3.0/|Creative Commons BY-NC-SA 3.0]].</WRAP>+<tab>
  
 +====== Laminar flow hood ======
 +The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures.
 ====== Prototype 1 & 2 ====== ====== Prototype 1 & 2 ======
 The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below.
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 |Fan (Fasco™ A1200 + bracket)|  1  |  450  |  **450**  | |Front doors|  2  |  ?  |  **?**  | |Fan (Fasco™ A1200 + bracket)|  1  |  450  |  **450**  | |Front doors|  2  |  ?  |  **?**  |
 |HEPA Filter (18"x24"x6”)|  1  |  175  |  **175**  | |Glass work area (24"x23"x¼”)|  1  |  30  |  **30**  | |HEPA Filter (18"x24"x6”)|  1  |  175  |  **175**  | |Glass work area (24"x23"x¼”)|  1  |  30  |  **30**  |
-|Pre-filters (9 ¾"x19 ¾")|  2  |  5.75  |  **11.5**  | |Glass shelves (4 ½"x12"x1/8")|  2  |  7  |  **14**  |+|Pre-filters (9 ¾"x19 ¾")|  2  |  5.75  |  **11.5**  | |Glass shelves (4 ½"x12"x¼")|  2  |  7  |  **14**  |
 |3-positions switch (115V, 15A)|  1  |  6  |  **6**  | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]|  4  |  3.5  |  **14**  | |3-positions switch (115V, 15A)|  1  |  6  |  **6**  | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]|  4  |  3.5  |  **14**  |
 |Power cord (115V, 15A)|  1  |  10  |  **10**  | | | | ^  **58**  | |Power cord (115V, 15A)|  1  |  10  |  **10**  | | | | ^  **58**  |
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