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en:construction:hotte_a_flux_laminaire [2013/04/03 22:19] ph [Overview of cuts] |
en:construction:hotte_a_flux_laminaire [2018/06/27 15:20] (current) |
<WRAP hide>====== Laminar flow hood</WRAP> | ~~META:title=Laminar flow hood~~ |
<WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=6HLVNSG9CPQYJ|financial contribution]], regardless of the amount, would be greatly appreciated. | <wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish at the time the lid is opened; during pouring, inoculation or incubation »</wrap>[(:ref:stamets_ggm)] |
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Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http://creativecommons.org/licenses/by-nc-sa/3.0/|Creative Commons BY-NC-SA 3.0]]. Make good use of it.</WRAP> | <tab> |
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| ====== Presentation ====== |
| The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures. |
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====== Prototype 1 & 2 ====== | ====== Prototype 1 & 2 ====== |
The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. | The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. |
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<WRAP box 65% center>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> | <WRAP centeralign>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> |
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<WRAP box 61% center>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> | |
<WRAP clear></WRAP> | |
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| <WRAP centeralign>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> |
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===== Improvements ===== | ===== Improvements ===== |
| **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | | | **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | |
| **2** |Stirrer-motors | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | | | **2** |Stirrer-motors | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | |
| **4** |Stirrer-front stands | **SFb** | 1 x 5 | 2.54 x 12.7 | | | **6** |Stirrer-front stands | **SFb** | 1 x 5 | 2.54 x 12.7 | |
| **2** |Stirrer-rear stands | **SFc** | 1 x 2 | 2.54 x 5.08 | | | **2** |Stirrer-rear stands | **SFc** | 1 x 2 | 2.54 x 5.08 | |
</WRAP><WRAP column 45% right> | </WRAP><WRAP column 45% right> |
|Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | | |Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | |
|HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | | |HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | |
|Pre-filters (9 ¾"x19 ¾")| 2 | 5.75 | **11.5** | |Glass shelves (4 ½"x12"x1/8")| 2 | 7 | **14** | | |Pre-filters (9 ¾"x19 ¾")| 2 | 5.75 | **11.5** | |Glass shelves (4 ½"x12"x¼")| 2 | 7 | **14** | |
|3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 3.5 | **14** | | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 3.5 | **14** | |
|Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **58** | | |Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **58** | |