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en:construction:hotte_a_flux_laminaire [2013/03/27 17:31]
ph [Materials and assembly] |
en:construction:hotte_a_flux_laminaire [2018/06/27 15:20] (current)
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| <WRAP hide>====== Laminar flow hood</WRAP> | ~~META:title=Laminar flow hood~~ |
| <WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=6HLVNSG9CPQYJ|financial contribution]], regardless of the amount, would be greatly appreciated. | <wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish at the time the lid is opened; during pouring, inoculation or incubation »</wrap>[(:ref:stamets_ggm)] |
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| Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http://creativecommons.org/licenses/by-nc-sa/3.0/|Creative Commons BY-NC-SA 3.0]]. Make good use of it.</WRAP> | <tab> |
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| | ====== Presentation ====== |
| | The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures. |
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| ====== Prototype 1 & 2 ====== | ====== Prototype 1 & 2 ====== |
| The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. | The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. |
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| <WRAP box 65% center>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> | <WRAP centeralign>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> |
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| <WRAP box 61% center>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> | |
| <WRAP clear></WRAP> | |
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| | <WRAP centeralign>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> |
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| ===== Improvements ===== | ===== Improvements ===== |
| * To avoid cracking of the plywood, pre-drill the screw holes with a bit of a smaller diameter. | * To avoid cracking of the plywood, pre-drill the screw holes with a bit of a smaller diameter. |
| * Seal well all the internal parts of the hood by drawing a silicone seal. The junction between the two parts (filter and engine), and the periphery of the prefilters are sealed with packing tape, much easier to remove than silicone. Each pre-filter is individually sealed to the support. | * Seal well all the internal parts of the hood by drawing a silicone seal. The junction between the two parts (filter and engine), and the periphery of the prefilters are sealed with packing tape, much easier to remove than silicone. Each pre-filter is individually sealed to the support. |
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| | <tab>{{:fr:construction:montage1.jpg?100|}} {{:fr:construction:montage2.jpg?100|}} {{:fr:construction:montage3.jpg?160|}} {{:fr:construction:montage4.jpg?160|}} |
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| **Tools needed** | **Tools needed** |
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| ===== Magnetic stirrers ===== | ===== Magnetic stirrers ===== |
| Commercial magnetic stirrers are large, expensive, and often stronger than necessary. To stir one to two liters, you can build one simply by sticking two hard drive magnets[(:hidden:>{{:fr:construction:aimants.png?200|}})] on a coupler mounted on a DC motor. The result, very compact, is easy to integrate directly below the work area. | <WRAP column 250px><tab>{{:fr:construction:stirrer0.jpg?200|}}</WRAP>Commercial magnetic stirrers are large, expensive, and often stronger than necessary. To stir one to two liters, you can build one simply by sticking two hard drive magnets[(:hidden:>{{:fr:construction:aimants.png?200|}})] on a coupler mounted on a DC motor. The result, very compact, is easy to integrate directly below the work area. |
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| | * Align SF to the center of DF and tighten with clamps. Screw the two pieces together and remove the clamps. As shown, align the two SFa on DF, place the clamps, screw them, then remove the clamps. SF, DF and SFa 1-2 are now linked in a single piece. |
| | * Drill 1 1/2" holes through the thickness of SF and 5/8" holes through the thickness of DF+SFa+SFa. |
| | * Add the legs SFb and SFc, then prepare the stirrers; |
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| * Start by picking up the magnets from an old hard drive. You will probably need a special screwdriver (torx). Each magnet is mounted on a metal plate. To recover it, bend the plate in the opposite direction to the magnet. | * Pick up the magnets from an old hard drive. You will probably need a special screwdriver (torx). Each magnet is mounted on a metal plate. To recover it, bend the plate in the opposite direction to the magnet. |
| * Join two magnets together, then preheat the magnets and the coupler (with a hot air gun or a bain-marie...). Meanwhile, preheat a hot glue gun. | * Join two magnets together, then preheat the magnets and the coupler (with a hot air gun or a bain-marie...). Meanwhile, preheat a hot glue gun. |
| * Put some glue on the coupler and attach it to the magnets, well centered. Leave to dry. | * Put some glue on the coupler and attach it to the magnets, well centered. Leave to dry. |
| * Place the assembly on a motor, then secure the coupler by tightening the screw against the shaft. | * Place the assembly on a motor, then secure the coupler by tightening the screw against the shaft. |
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| | * Place the motors into the previously drilled holes. |
| * Solder the wires to the poles of the motor, as shown in the [[en:construction:hotte_a_flux_laminaire&#wiring_diagram|wiring diagram]]. | * Solder the wires to the poles of the motor, as shown in the [[en:construction:hotte_a_flux_laminaire&#wiring_diagram|wiring diagram]]. |
| * Drill SF (as shown below). | * In order to keep a space of 1/16" between the magnets and the surface of SF, place a cardboard piece of 1/16" thick on a straight board, and push it against the magnets. |
| * Align SF, DF and SFa with the holes, screw the pieces together and drill them (as shown) to place the engines. | * Place a piece of electrical tape onto the wire end of the motors and secure it with hot glue. |
| * Place a piece of styrofoam between the magnets and DF (temporarily) and make sure that there is a space left between the top of the magnet and the surface of SF. Then fill that space with another piece of styrofoam. | * Solder the wires to the 6-pin female plug and insulate the connections. |
| * Stick the magnets to the surface with some tape, turn the screwed sheets (SF + DF + SFa) upside down, pull the wires out between the two SFa, and secure the engines with hot glue. | |
| * Turn over the sheets, remove the tape and the styrofoam, and complete the wiring. | |
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| | <tab>{{:fr:construction:colle1.jpg?100|}} {{:fr:construction:colle2.jpg?100|}} {{:fr:construction:colle3.jpg?100|}} {{:fr:construction:colle3b.jpg?100|}} {{:fr:construction:colle4.jpg?100|}} {{:fr:construction:stirrer1.jpg?100|}} {{:fr:construction:stirrer2.jpg?100|}} |
| ===== Maintenance ===== | ===== Maintenance ===== |
| **Cleaning** | **Cleaning** |
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| Before each use, the internal surfaces of the work area should be cleaned with 70% isopropyl alcohol. The "floor" must be particularly clean. Pre-filters are cleaned with a vacuum cleaner, if needed. | Before each use, the internal surfaces of the work area should be cleaned with 70% isopropyl alcohol. The "floor" must be particularly clean. Pre-filters are cleaned with a vacuum cleaner, if needed. |
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| {{:fr:construction:nettoyage.png?100|}} | |
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| **Lubrication** | **Lubrication** |
| The motor should be oiled once a year with a suitable lubricant. With a screwdriver, remove the cap in the center of the fan and apply a few drops of sewing machine oil. | The motor should be oiled once a year with a suitable lubricant. With a screwdriver, remove the cap in the center of the fan and apply a few drops of sewing machine oil. |
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| {{:fr:construction:huilage.png?100|}} | {{:fr:construction:nettoyage.png?100|}} {{:fr:construction:huilage.png?100|}} |
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| ====== Plans cuts ====== | ====== Plans cuts ====== |
| | **1** |Rear | **AF** | 18 x 24 | 45.72 x 60.96 | | | **1** |Rear | **AF** | 18 x 24 | 45.72 x 60.96 | |
| | **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | | | **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | |
| | **2** |Stirrer-a | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | | | **2** |Stirrer-motors | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | |
| | **4** |Stirrer-b | **SFb** | 1 x 5 | 2.54 x 12.7 | | | **6** |Stirrer-front stands | **SFb** | 1 x 5 | 2.54 x 12.7 | |
| | | **2** |Stirrer-rear stands | **SFc** | 1 x 2 | 2.54 x 5.08 | |
| </WRAP><WRAP column 45% right> | </WRAP><WRAP column 45% right> |
| **Motor (M)** | **Motor (M)** |
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| The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. | The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. |
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| | {{:fr:construction:dc1.jpg?100|}} {{:fr:construction:dc2.jpg?100|}} {{:fr:construction:dc3.jpg?100|}} |
| ====== Budget ====== | ====== Budget ====== |
| Most parts are easily found in hardware stores. The electronics come from [[http://ebay.ca|Ebay Canada]], the HEPA filter from [[http://fungi.com|Fungi Perfecti]] and the 6-pin connector from [[https://www.sparkfun.com/products/11475|Sparkfun]]. | Most parts are easily found in hardware stores. The electronics come from [[http://ebay.ca|Ebay Canada]], the HEPA filter from [[http://fungi.com|Fungi Perfecti]] and the 6-pin connector from [[https://www.sparkfun.com/products/11475|Sparkfun]]. |
| |Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | | |Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | |
| |HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | | |HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | |
| |Pre-filters (9 ¾"x19 ¾")| 2 | 7 | **14** | |Glass shelves (4 ½"x12"x1/8")| 2 | 7 | **14** | | |Pre-filters (9 ¾"x19 ¾")| 2 | 5.75 | **11.5** | |Glass shelves (4 ½"x12"x¼")| 2 | 7 | **14** | |
| |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass Shelf Clip[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 6 | **24** | | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 3.5 | **14** | |
| |Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **68** | | |Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **58** | |
| |AC plug (115V, 15A)[(:hidden:>{{:fr:construction:ac_plug.jpg?100|}})]| 1 | 5 | **5** | ^ **Electronic** ^ **u** ^ **$/u** ^ **$** ^ | |AC plug (115V, 15A)[(:hidden:>{{:fr:construction:ac_plug.jpg?100|}})]| 1 | 5 | **5** | ^ **Electronics** ^ **u** ^ **$/u** ^ **$** ^ |
| |Plywood (quality select, 4'x8'x¾”) | 2 | 40 | **80** | |Switching Power Supply[(:hidden:>{{:fr:construction:power_supply.jpg?100|}})] (115V AC, 12V DC, 3A[(:hidden:>Amperage for all the modules shown (motors 4*0.5A + LED 0.6A = 2.6A))])| 1 | 11 | **11** | | |Plywood (quality select, 4'x8'x¾”) | 2 | 40 | **80** | |Switching Power Supply[(:hidden:>{{:fr:construction:power_supply.jpg?100|}})] (115V AC, 12V DC, 3A[(:hidden:>Current for all the modules shown (motors 4*0.5A + LED 0.6A = 2.6A))])| 1 | 11 | **11** | |
| |Primer & epoxy paint (for 128ft²)| 1 | 50 | **50** | |Dual Switch Wall (115V, 15A)[(:hidden:>{{:fr:construction:dual_switch.jpg?100|}})]| 1 | 7 | **7** | | |Primer (1L) & epoxy paint (2L) | 1 | - | **50** | |Dual Switch Wall (115V, 15A)[(:hidden:>{{:fr:construction:dual_switch.jpg?100|}})]| 1 | 7 | **7** | |
| |Handles| 6 | 18 | **108** | |LED Strip (50cm, 30 LED, 12V, 0.6A, waterproof)[(:hidden:>{{:fr:construction:led_strip.jpg?100|}})]| 1 | 3 | **3** | | |Handles| 6 | 18 | **108** | |LED Strip (50cm, 30 LED, 12V, 0.6A, waterproof)[(:hidden:>{{:fr:construction:led_strip.jpg?100|}})]| 1 | 3 | **3** | |
| |Latches| 3 | 12 | **36** | |6-Pin Chassis Connector[(:hidden:>{{:fr:construction:6pin2.jpg?100|Image from Sparkfun.com}})] | 1 | 11 | **11** | | |Latches| 2 | 4 | **8** | |6-Pin Chassis Connector[(:hidden:>{{:fr:construction:6pin2.jpg?100|Image provenant de Sparkfun.com}})] | 1 | 11 | **11** | |
| |Screws ½”, ¾”, 1”, et 1 ½”| 4 | 4.5 | **18** | |4 Gang Button Wall Switch[(:hidden:>{{:fr:construction:4_button_switch.jpg?100|}})]| 1 | 7 | **7** | | |Screws ¾” et 1 ½”| 2 | 8 | **16** | |4 Gang Button Wall Switch[(:hidden:>{{:fr:construction:4_button_switch.jpg?100|}})]| 1 | 7 | **7** | |
| |Silicon| 1 | 6 | **6** | |Motors (12V, 0.5A, 200RPM, 3mm shaft)[(:hidden:>{{:fr:construction:dc_motor.jpg?100|}})]| 4 | 8 | **32** | | |Silicon| 1 | 6 | **6** | |Motors (12V, 0.5A, 200RPM, 3mm shaft)[(:hidden:>{{:fr:construction:dc_motor.jpg?100|}})]| 4 | 8 | **32** | |
| |Tuck™ tape| 1 | 6 | **6** | |Shaft Coupler (3mm)[(:hidden:>{{:fr:construction:coupleur.jpg?100|}})]| 4 | 6 | **24** | | |Packing tape| 1 | 2 | **2** | |Shaft Coupler (3mm)[(:hidden:>{{:fr:construction:coupleur.jpg?100|}})]| 4 | 6 | **24** | |
| |Paint brush, roller, tray & sleeve| 1 | 9 | **9** | |Wire (16AWG, 2 pin, red & black)| 1 | ? | **4** | | |Paint brush, roller, tray & sleeve| 1 | 9 | **9** | |Wire (16AWG, 2 pin, red and black)| 1 | ? | **4** | |
| | | | ^ **973** | | | | ^ **99** | | |Hooks| 2 | 2.5 | **5** | | | | ^ **99** | |
| | | | | ^ **942** | | | | | | |
| **Total: 1140** | **Total: 1140** |
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| <tab> | |
| ---- | |
| <WRAP lo>Note: Most of the pictures of the electronic components shown in the budget section come from Ebay Canada.</WRAP> | |