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en:construction:hotte_a_flux_laminaire [2013/04/02 22:06]
ph [Materials and assembly] |
en:construction:hotte_a_flux_laminaire [2018/06/27 15:20] (current)
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| <WRAP hide>====== Laminar flow hood</WRAP> | ~~META:title=Laminar flow hood~~ |
| <WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=6HLVNSG9CPQYJ|financial contribution]], regardless of the amount, would be greatly appreciated. | <wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish at the time the lid is opened; during pouring, inoculation or incubation »</wrap>[(:ref:stamets_ggm)] |
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| Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http://creativecommons.org/licenses/by-nc-sa/3.0/|Creative Commons BY-NC-SA 3.0]]. Make good use of it.</WRAP> | <tab> |
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| | ====== Presentation ====== |
| | The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures. |
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| ====== Prototype 1 & 2 ====== | ====== Prototype 1 & 2 ====== |
| The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. | The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. |
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| <WRAP box 65% center>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> | <WRAP centeralign>{{:fr:construction:prototype1.jpg?88|Prototype 1}}{{:fr:construction:p3.jpg?100|}}{{:fr:construction:p4.jpg?100|}}{{:fr:construction:p5.jpg?100|}}{{:fr:construction:p6.jpg?100|}}{{:fr:construction:p7.jpg?100|}}</WRAP> |
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| <WRAP box 61% center>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> | |
| <WRAP clear></WRAP> | |
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| | <WRAP centeralign>{{:fr:construction:p1.jpg?152|}}{{:fr:construction:p2.jpg?85|}}{{:fr:construction:p9-1.jpg?150|}}{{:fr:construction:p9.jpg?170|}}</WRAP> |
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| ===== Improvements ===== | ===== Improvements ===== |
| | **1** |Rear | **AF** | 18 x 24 | 45.72 x 60.96 | | | **1** |Rear | **AF** | 18 x 24 | 45.72 x 60.96 | |
| | **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | | | **1** |Stirrer | **SF** | 23 x 24 | 58.42 x 60.96 | |
| | **2** |Stirrer-a | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | | | **2** |Stirrer-motors | **SFa** | 2 x 25 ½ | 5.08 x 64.77 | |
| | **4** |Stirrer-b | **SFb** | 1 x 5 | 2.54 x 12.7 | | | **6** |Stirrer-front stands | **SFb** | 1 x 5 | 2.54 x 12.7 | |
| | | **2** |Stirrer-rear stands | **SFc** | 1 x 2 | 2.54 x 5.08 | |
| </WRAP><WRAP column 45% right> | </WRAP><WRAP column 45% right> |
| **Motor (M)** | **Motor (M)** |
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| The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. | The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. |
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| | {{:fr:construction:dc1.jpg?100|}} {{:fr:construction:dc2.jpg?100|}} {{:fr:construction:dc3.jpg?100|}} |
| ====== Budget ====== | ====== Budget ====== |
| Most parts are easily found in hardware stores. The electronics come from [[http://ebay.ca|Ebay Canada]], the HEPA filter from [[http://fungi.com|Fungi Perfecti]] and the 6-pin connector from [[https://www.sparkfun.com/products/11475|Sparkfun]]. | Most parts are easily found in hardware stores. The electronics come from [[http://ebay.ca|Ebay Canada]], the HEPA filter from [[http://fungi.com|Fungi Perfecti]] and the 6-pin connector from [[https://www.sparkfun.com/products/11475|Sparkfun]]. |
| |Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | | |Fan (Fasco™ A1200 + bracket)| 1 | 450 | **450** | |Front doors| 2 | ? | **?** | |
| |HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | | |HEPA Filter (18"x24"x6”)| 1 | 175 | **175** | |Glass work area (24"x23"x¼”)| 1 | 30 | **30** | |
| |Pre-filters (9 ¾"x19 ¾")| 2 | 7 | **14** | |Glass shelves (4 ½"x12"x1/8")| 2 | 7 | **14** | | |Pre-filters (9 ¾"x19 ¾")| 2 | 5.75 | **11.5** | |Glass shelves (4 ½"x12"x¼")| 2 | 7 | **14** | |
| |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass Shelf Clip[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 6 | **24** | | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]| 4 | 3.5 | **14** | |
| |Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **68** | | |Power cord (115V, 15A)| 1 | 10 | **10** | | | | ^ **58** | |
| |AC plug (115V, 15A)[(:hidden:>{{:fr:construction:ac_plug.jpg?100|}})]| 1 | 5 | **5** | ^ **Electronic** ^ **u** ^ **$/u** ^ **$** ^ | |AC plug (115V, 15A)[(:hidden:>{{:fr:construction:ac_plug.jpg?100|}})]| 1 | 5 | **5** | ^ **Electronics** ^ **u** ^ **$/u** ^ **$** ^ |
| |Plywood (quality select, 4'x8'x¾”) | 2 | 40 | **80** | |Switching Power Supply[(:hidden:>{{:fr:construction:power_supply.jpg?100|}})] (115V AC, 12V DC, 3A[(:hidden:>Amperage for all the modules shown (motors 4*0.5A + LED 0.6A = 2.6A))])| 1 | 11 | **11** | | |Plywood (quality select, 4'x8'x¾”) | 2 | 40 | **80** | |Switching Power Supply[(:hidden:>{{:fr:construction:power_supply.jpg?100|}})] (115V AC, 12V DC, 3A[(:hidden:>Current for all the modules shown (motors 4*0.5A + LED 0.6A = 2.6A))])| 1 | 11 | **11** | |
| |Primer & epoxy paint (for 128ft²)| 1 | 50 | **50** | |Dual Switch Wall (115V, 15A)[(:hidden:>{{:fr:construction:dual_switch.jpg?100|}})]| 1 | 7 | **7** | | |Primer (1L) & epoxy paint (2L) | 1 | - | **50** | |Dual Switch Wall (115V, 15A)[(:hidden:>{{:fr:construction:dual_switch.jpg?100|}})]| 1 | 7 | **7** | |
| |Handles| 6 | 18 | **108** | |LED Strip (50cm, 30 LED, 12V, 0.6A, waterproof)[(:hidden:>{{:fr:construction:led_strip.jpg?100|}})]| 1 | 3 | **3** | | |Handles| 6 | 18 | **108** | |LED Strip (50cm, 30 LED, 12V, 0.6A, waterproof)[(:hidden:>{{:fr:construction:led_strip.jpg?100|}})]| 1 | 3 | **3** | |
| |Latches| 3 | 12 | **36** | |6-Pin Chassis Connector[(:hidden:>{{:fr:construction:6pin2.jpg?100|Image from Sparkfun.com}})] | 1 | 11 | **11** | | |Latches| 2 | 4 | **8** | |6-Pin Chassis Connector[(:hidden:>{{:fr:construction:6pin2.jpg?100|Image provenant de Sparkfun.com}})] | 1 | 11 | **11** | |
| |Screws ½”, ¾”, 1”, et 1 ½”| 4 | 4.5 | **18** | |4 Gang Button Wall Switch[(:hidden:>{{:fr:construction:4_button_switch.jpg?100|}})]| 1 | 7 | **7** | | |Screws ¾” et 1 ½”| 2 | 8 | **16** | |4 Gang Button Wall Switch[(:hidden:>{{:fr:construction:4_button_switch.jpg?100|}})]| 1 | 7 | **7** | |
| |Silicon| 1 | 6 | **6** | |Motors (12V, 0.5A, 200RPM, 3mm shaft)[(:hidden:>{{:fr:construction:dc_motor.jpg?100|}})]| 4 | 8 | **32** | | |Silicon| 1 | 6 | **6** | |Motors (12V, 0.5A, 200RPM, 3mm shaft)[(:hidden:>{{:fr:construction:dc_motor.jpg?100|}})]| 4 | 8 | **32** | |
| |Tuck™ tape| 1 | 6 | **6** | |Shaft Coupler (3mm)[(:hidden:>{{:fr:construction:coupleur.jpg?100|}})]| 4 | 6 | **24** | | |Packing tape| 1 | 2 | **2** | |Shaft Coupler (3mm)[(:hidden:>{{:fr:construction:coupleur.jpg?100|}})]| 4 | 6 | **24** | |
| |Paint brush, roller, tray & sleeve| 1 | 9 | **9** | |Wire (16AWG, 2 pin, red & black)| 1 | ? | **4** | | |Paint brush, roller, tray & sleeve| 1 | 9 | **9** | |Wire (16AWG, 2 pin, red and black)| 1 | ? | **4** | |
| | | | ^ **973** | | | | ^ **99** | | |Hooks| 2 | 2.5 | **5** | | | | ^ **99** | |
| | | | | ^ **942** | | | | | | |
| **Total: 1140** | **Total: 1140** |
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| <tab> | |
| ---- | |
| <WRAP lo>Note: Most of the pictures of the electronic components shown in the budget section come from Ebay Canada.</WRAP> | |