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en:construction:hotte_a_flux_laminaire [2013/03/28 11:28] ph [Magnetic stirrers] |
en:construction:hotte_a_flux_laminaire [2013/04/27 11:47] ph |
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- | <WRAP hide>====== Laminar flow hood</ | + | <wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish at the time the lid is opened; during pouring, inoculation or incubation »</ |
- | <WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https:// | + | |
- | Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http:// | + | <tab> |
+ | ====== Laminar flow hood ====== | ||
+ | The flow hood is a sterile air blower. The particles of the air are separated by a pre-filter and a HEPA filter. This results in almost pure air, separated at 99.97% from particles greater than 0.3 µm. This clean air exerts a positive pressure on the work plan, allowing to easily produce axenic cultures. | ||
====== Prototype 1 & 2 ====== | ====== Prototype 1 & 2 ====== | ||
The first prototype met the expectations, | The first prototype met the expectations, | ||
- | < | + | < |
- | + | ||
- | <WRAP box 61% center> | + | |
- | <WRAP clear></ | + | |
+ | <WRAP centeralign> | ||
===== Improvements ===== | ===== Improvements ===== | ||
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* To avoid cracking of the plywood, pre-drill the screw holes with a bit of a smaller diameter. | * To avoid cracking of the plywood, pre-drill the screw holes with a bit of a smaller diameter. | ||
* Seal well all the internal parts of the hood by drawing a silicone seal. The junction between the two parts (filter and engine), and the periphery of the prefilters are sealed with packing tape, much easier to remove than silicone. Each pre-filter is individually sealed to the support. | * Seal well all the internal parts of the hood by drawing a silicone seal. The junction between the two parts (filter and engine), and the periphery of the prefilters are sealed with packing tape, much easier to remove than silicone. Each pre-filter is individually sealed to the support. | ||
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+ | < | ||
**Tools needed** | **Tools needed** | ||
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===== Magnetic stirrers ===== | ===== Magnetic stirrers ===== | ||
- | Commercial magnetic stirrers are large, expensive, and often stronger than necessary. To stir one to two liters, you can build one simply by sticking two hard drive magnets[(: | + | <WRAP column 250px>< |
* Align SF to the center of DF and tighten with clamps. Screw the two pieces together and remove the clamps. As shown, align the two SFa on DF, place the clamps, screw them, then remove the clamps. SF, DF and SFa 1-2 are now linked in a single piece. | * Align SF to the center of DF and tighten with clamps. Screw the two pieces together and remove the clamps. As shown, align the two SFa on DF, place the clamps, screw them, then remove the clamps. SF, DF and SFa 1-2 are now linked in a single piece. | ||
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* Place a piece of electrical tape onto the wire end of the motors and secure it with hot glue. | * Place a piece of electrical tape onto the wire end of the motors and secure it with hot glue. | ||
* Solder the wires to the 6-pin female plug and insulate the connections. | * Solder the wires to the 6-pin female plug and insulate the connections. | ||
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+ | < | ||
===== Maintenance ===== | ===== Maintenance ===== | ||
**Cleaning** | **Cleaning** | ||
Before each use, the internal surfaces of the work area should be cleaned with 70% isopropyl alcohol. The " | Before each use, the internal surfaces of the work area should be cleaned with 70% isopropyl alcohol. The " | ||
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**Lubrication** | **Lubrication** | ||
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The motor should be oiled once a year with a suitable lubricant. With a screwdriver, | The motor should be oiled once a year with a suitable lubricant. With a screwdriver, | ||
- | {{: | + | {{: |
====== Plans cuts ====== | ====== Plans cuts ====== | ||
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| **1** |Rear | **AF** | | **1** |Rear | **AF** | ||
| **1** |Stirrer | | **1** |Stirrer | ||
- | | **2** |Stirrer-a | + | | **2** |Stirrer-motors |
- | | **4** |Stirrer-b | + | | **6** |Stirrer-front stands |
+ | | **2** |Stirrer-rear stands | **SFc** | ||
</ | </ | ||
**Motor (M)** | **Motor (M)** | ||
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The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. | The three-position switch allow fan speed selection. It may be replaced by a two-position switch, set to the highest speed. | ||
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+ | {{: | ||
====== Budget ====== | ====== Budget ====== | ||
Most parts are easily found in hardware stores. The electronics come from [[http:// | Most parts are easily found in hardware stores. The electronics come from [[http:// | ||
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|Fan (Fasco™ A1200 + bracket)| | |Fan (Fasco™ A1200 + bracket)| | ||
|HEPA Filter (18" | |HEPA Filter (18" | ||
- | |Pre-filters (9 ¾"x19 ¾" | + | |Pre-filters (9 ¾"x19 ¾" |
- | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass | + | |3-positions switch (115V, 15A)| 1 | 6 | **6** | |Glass |
- | |Power cord (115V, 15A)| 1 | 10 | **10** | + | |Power cord (115V, 15A)| 1 | 10 | **10** |
- | |AC plug (115V, 15A)[(: | + | |AC plug (115V, 15A)[(: |
- | |Plywood (quality select, 4' | + | |Plywood (quality select, 4' |
- | |Primer & epoxy paint (for 128ft²)| 1 | | + | |Primer |
|Handles| | |Handles| | ||
- | |Latches| | + | |Latches| |
- | |Screws | + | |Screws ¾” et 1 ½”| |
|Silicon| | |Silicon| | ||
- | |Tuck™ | + | |Packing |
- | |Paint brush, roller, tray & sleeve| | + | |Paint brush, roller, tray & sleeve| |
- | | | | ^ **973** | | | | ^ **99** | + | |Hooks| |
+ | | | | ^ **942** | ||
**Total: 1140** | **Total: 1140** | ||
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- | <tab> | ||
- | ---- | ||
- | <WRAP lo>Note: Most of the pictures of the electronic components shown in the budget section come from Ebay Canada.</ |