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Introduction

— Home
— Introduction

Agar culture

— Preparation
— Transplanting
— Culture of spores, storage

Indoor cultivation

Vegetative
— Substrate preparation
— Liquid culture, incubation

Generative
— Flowering, harvest
— Spores harvest

Outdoor cultivation

— Stumps and logs
— Establishment of a parcel
— Propagation
— Harvest

Construction

★ Laminar flow hood
— Flask for liquid culture
— Blooming box
— Bioreactor

Appendices

★ Recipes calculator
— Equipement and suppliers

About

★ Donate

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en:construction:hotte_a_flux_laminaire [2013/04/09 21:04]
ph 		en:construction:hotte_a_flux_laminaire [2013/04/23 14:08]
ph [Laminar flow hood]
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-<WRAP hide>====== Laminar flow hood</WRAP> +<wrap lo>« Air can be described as a sea of invisible micro-organisms, that directly contaminate sterilized media once exposed. Airborne contaminants enter a petri dish either at the time the lid is opened (during pouring or inoculation) or during incubation »</wrap>[(:ref:stamets_ggm)]
-<WRAP info>The plans presented below have required many hours of work, and some expensive trials and errors. If you find they were useful for you, a [[https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=6HLVNSG9CPQYJ|financial contribution]], regardless of the amount, would be greatly appreciated.+
  
-Please note that like all the unreferenced information on this wiki, this content is __only__ available __under the terms of the license__ [[http://creativecommons.org/licenses/by-nc-sa/3.0/|Creative Commons BY-NC-SA 3.0]].</WRAP>+<tab>
  
 +====== Laminar flow hood ======
 +The flow hood is a sterile air blower. The particles are separated from the ambient air by a prefilter, then by an HEPA filter. This results in clean air at 99.97%, which exerts a positive pressure on the work plan.
 ====== Prototype 1 & 2 ====== ====== Prototype 1 & 2 ======
 The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below. The first prototype met the expectations, but several details deserved to be improved. The plans, drawings and the budget of the second version are available below.
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 |Fan (Fasco™ A1200 + bracket)|  1  |  450  |  **450**  | |Front doors|  2  |  ?  |  **?**  | |Fan (Fasco™ A1200 + bracket)|  1  |  450  |  **450**  | |Front doors|  2  |  ?  |  **?**  |
 |HEPA Filter (18"x24"x6”)|  1  |  175  |  **175**  | |Glass work area (24"x23"x¼”)|  1  |  30  |  **30**  | |HEPA Filter (18"x24"x6”)|  1  |  175  |  **175**  | |Glass work area (24"x23"x¼”)|  1  |  30  |  **30**  |
-|Pre-filters (9 ¾"x19 ¾")|  2  |  5.75  |  **11.5**  | |Glass shelves (4 ½"x12"x1/8")|  2  |  7  |  **14**  |+|Pre-filters (9 ¾"x19 ¾")|  2  |  5.75  |  **11.5**  | |Glass shelves (4 ½"x12"x¼")|  2  |  7  |  **14**  |
 |3-positions switch (115V, 15A)|  1  |  6  |  **6**  | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]|  4  |  3.5  |  **14**  | |3-positions switch (115V, 15A)|  1  |  6  |  **6**  | |Glass shelf clips[(:hidden:>{{:fr:construction:glass_clip.jpg?100|}})]|  4  |  3.5  |  **14**  |
 |Power cord (115V, 15A)|  1  |  10  |  **10**  | | | | ^  **58**  | |Power cord (115V, 15A)|  1  |  10  |  **10**  | | | | ^  **58**  |
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